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AIF1/IBA1 isoform 1 and 3 Antibody
SKU: orb18542
Description
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:128000, WB: 0.5-1μg/ml, IHC-P: 5μg/ml |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Porcine |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | AIF1/IBA1 isoform 1 and 3 |
| Molecular Weight | 10.5; 16.7 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Disclaimer | For research use only |
Alternative Names
−AIF1; IBLY409840A1; allograft inflammatory factor 1; AIF-1; IRT-1; interferon gamma responsive transcript; ionized calcium-binding adapter molecule; Daintain; DADB-70P7.8
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1 µg/ml staining of Mouse (A) and (3 µg/ml) Rat (B) Brain lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
6 µg/ml staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
Negative Control showing staining of paraffin embedded Human Frontal Cortex, with no primary antibody.
4 µg/ml staining of paraffin embedded Human Frontal Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Song-I Seol 1, Sang-A Oh 1, Dashdulam Davaanyam 1, Ja-Kyeong Lee Blocking peptidyl arginine deiminase 4 confers neuroprotective effect in the post-ischemic brain through both NETosis-dependent and -independent mechanisms Acta Neuropathol Commun, (2025)
Applications
IF
Reactivity
Rat
AIF1/IBA1 isoform 1 and 3 Antibody (orb18542)
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