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B7-H4 Antibody
SKU: orb19600
Description
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, FC, IF, WB |
|---|---|
| Dilution range | ELISA: 1:64000, WB: 0.1-1 μg/ml, IHC-P: 10ug/ml |
| Reactivity | Human |
| Predicted Reactivity | Canine, Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | B7-H4 |
| Molecular Weight | 30.9; 20.7 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Disclaimer | For research use only |
Alternative Names
−anti B7-H4 antibody, anti B7H4 antibody, anti B7S1 antibody, anti B7X antibody, anti B7h.5 antibody, anti FLJ22418 antibody, anti PRO1291 antibody, anti RP11-229A19.4 antibody, anti VCTN1 antibody, anti T cell costimulatory molecule B7x antibody, anti immune costimulatory protein antibody, anti V-set domain containing T cell activation inhibitor 1 antibody
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Flow cytometric analysis of paraformaldehyde fixed MCF7 cells using VTCN1 antibody.
Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells using VTCN1 antibody.
Western blot analysis of HEK293 iso 1 + iso 2 cell lysate (lane 1) + peptide (lane 2). Human Adrenal Gland iso 1 (lane 3), Human Adrenal Gland iso 2 (lane 4) and Human pancreas iso 1 (lane 5) lysate using VTCN1 antibody.
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
B7-H4 Antibody (orb19600)
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