DEAD-box protein 6 Antibody

SKU: orb19176

Description

Goat polyclonal antibody to DDX6

Research Area

Cancer, Epigenetics

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IHC, WB
Dilution Range	ELISA: 1:128000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
Reactivity	Human
Predicted Reactivity	Canine, Mouse, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 50kDa band observed in lysates of cell line Jurkat (calculated MW of 53.2kDa according to NP_NP_004388.1). In transfected HEK293 transiently expressing Human DDX6 a band of approx. 55kDa is observed. This band is not observed in the non-transfected HEK293. The endogenous 53kDa protein is observed in both. Recommended concentration: 0.1-0.3 μg/ml. Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	DEAD-box protein 6
Protein Sequence	AEYHSEPVEDEKP
Molecular Weight	53.2
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti DEAD-box protien 6 antibody, anti DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 antibody, anti DDX6 antibody, anti HLR2 antibody, anti P54 antibody, anti RCK antibody, anti DEAD box-6 antibody, anti DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD) antibody

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Western blot analysis of staining of Daudi (A), Jurkat (B) and HepG2 (C) lysates (35µg protein in RIPA buffer) using DDX6 antibody.

Immunohistochemical staining of Human Cortex using DDX6 antibody

Immunohistochemical staining of Human Thymus using DDX6 antibody

Quick Database Links

Gene Symbol

DEAD-box protein 6

NCBI Gene ID

1656

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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