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Goat anti-Mouse IgG (H&L), F(ab)'2 fragment, HRP conjugated

SKU: orb700423

Description

Goat polyclonal antibody against Goat anti-Mouse IgG (H&L) conjugated to HRP

Images & Validation

Dilution range1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)
ReactivityMouse
Application Notes
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.

Key Properties

HostGoat
ClonalityPolyclonal
PurityImmunogen affinity purified goat IgG, F(ab)'2 fragment.
PurificationThis antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
ConjugationHRP

Storage & Handling

StorageStore non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Form/AppearanceLiquid
DisclaimerFor research use only

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Goat anti-Mouse IgG (H&L), F(ab)'2 fragment, HRP conjugated (orb700423)

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0.5 mg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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