Goat IgG (H&L) Antibody Biotin Conjugated Pre-Adsorbed

SKU: orb347036

Description

Goat IgG (H&L) antibody (Biotin)

Images & Validation

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Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:600,000, IHC: 1:1,000 - 1:5,000, WB: 1:60,000
Reactivity	Goat
Application Notes	Goat IgG Biotin Conjugated Antibody has been tested by ELISA and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.

Key Properties

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Antibody Type	Secondary Antibody
Host	Donkey
Clonality	Polyclonal
Isotype	IgG
Immunogen	Anti-Goat IgG (H&L) was produced by repeated immunization with goat IgG whole molecule in donkey.
Purity	Anti-Goat IgG (H&L) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against Chicken, Guinea Pig, Horse, Hamster, Mouse, Rabbit and Rat Serum Proteins.
Conjugation	Biotin

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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donkey anti-Goat IgG Antibody biotin Conjugation, donkey anti-Goat IgG biotin Conjugated Antibody

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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