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Featured
KO/KD
Validated
Validated
Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC, KO/KD Validated, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1160 to the C-terminus of human HER2 / ErbB2 (NP_004439.2). |
| Target | ErbB2 |
| Molecular Weight | Observed: 185kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−erbB-2 Antibody: NEU, NGL, HER2, TKR1, CD340, HER-2, MLN 19, HER-2/neu, MLN19, NEU, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein
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Western blot analysis of extracts of various cell lines, using HER2/ErbB2 antibody (orb1274469) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western blot analysis of extracts from normal (control) and HER2/ErbB2 knockout (KO) HeLa cells, using HER2/ErbB2 antibody (orb1274469) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.
Immunohistochemistry of paraffin-embedded rat lung using HER2/ErbB2 antibody (orb1274469) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using HER2/ErbB2 antibody (orb1274469) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using HER2/ErbB2 antibody (orb1274469) at dilution of 1:100 (40x lens).
Quick Database Links
Gene Symbol
ErbB2
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ErbB2 Antibody (orb1274469)
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