HER2 Rabbit Polyclonal Antibody

SKU: orb1294317

Description

Rabbit polyclonal antibody to HER2

Research Area

Cancer Biology

Images & Validation

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Item 1 of 6

Tested Applications	IF, IHC, WB
Dilution Range	Western Blot 1:500–1:1000, Immunofluorescence 1:200–1:400, Immunohistochemistry (Paraffin) 1: 200-1:250
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit-IgG
Immunogen	Synthetic peptide / Intracellular domain corresponding to Human HER2 / ERBB2
Target	HER2 / ERBB2
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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All lanes: Anti-HER2 / ERBB2 antibody - at 1/500 dilution, Lysates/proteins at 30 µg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked with 3% BSA for an hour before being incubated with orb1294317 overnight at 4°C.

Application: Immunocytochemistry (ICC), Sample: Mixture cell, Antibody: HER2 / ERBB2 antibody (orb1294317).

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294317 anti-HER2 / ERBB2 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human Breast cancer tissue labeling HER2 / ERBB2 with orb1294317 at 1/200.

Quick Database Links

Gene Symbol

HER2 / ERBB2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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