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His-Tag Mouse mAb Antibody
SKU: orb763374
Description
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IP, WB |
|---|---|
| Dilution Range | WB 1:5,000 IP 1:200 IF 1:1,000 |
Key Properties
−| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Immunogen | Synthetic Peptide |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Concentration | 1mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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IP antibody use: 5ug His Mouse IgG1 per ml Lysate, WB 1:3000 1: untransfected 293 cell lysate 2: transfected 293 cell lysate with His-tag fusion protein 3: IP (untransfected 293 + anti-His mAb + Protein G agarose) 4: IP (transfected 293 + normal Mouse IgG + Prote).
IF analysis of 293 cells transfected with a His-tag protein, using ELKbio anti-HisTag Mouse mAb at a 1:1000 dilution (blue DAPI, red anti-His).
2ug His fusion protein + Primary antibody dilution at 1: 1:5, 000 2: 1:10, 000.
1: Multi-Tag Recombinant protein 2: 293T transfected with His recombinant protein 3: Insect cell trandfected with His recombinant protein.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
IP
Immunoprecipitation
His-Tag Mouse mAb Antibody (orb763374)
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