His tag Rabbit Polyclonal Antibody

SKU: orb1294276

Description

Rabbit polyclonal antibody to His tag

Images & Validation

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Item 1 of 1

Tested Applications	IF, IP, WB
Dilution Range	Western Blot 1:2000-1:4000, Immunofluorescence 1:500-1:1000

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit-IgG
Immunogen	KLH-conjugated HHHHHH (6X His)
Target	His tag
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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His-tag antibody detects His-tagged protein by western blot analysis. The membrane was then blocked for an hour before being incubated with orb1294276 overnight at 4°C. His-tag antibody (orb1294276) diluted at 1:2000.

Quick Database Links

Gene Symbol

His tag

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Datasheet

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Protocol Information

Western Blot (IB, immunoblot)

Immunofluorescence

Immunoprecipitation