Histone H4 rabbit pAb Antibody

SKU: orb767571

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Description

Rabbit polyclonal antibody to Histone H4

Images & Validation

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Tested Applications	ELISA, IF, IHC, WB
Dilution range	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Reactivity	Human, Monkey, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human Histone H4. AA range:6-55
Molecular Weight	11
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-HIST1H4A antibody, anti-H4/A antibody, anti-H4FA antibody, anti-HIST1H4B antibody, anti-H4/I antibody, anti-H4FI antibody, anti-HIST1H4C antibody, anti-H4/G antibody, anti-H4FG antibody, anti-HIST1H4D antibody, anti-H4/B antibody, anti-H4FB antibody, anti-HIST1H4E antibody, anti-H4/J antibody, anti-H4FJ antibody, anti-HIST1H4F antibody, anti-H4/C antibody, anti-H4FC antibody, anti-HIST1H4H antibody, anti-H4/H antibody, anti-H4FH antibody, anti-HIST1H4I antibody, anti-H4/M antibody, anti-H4FM antibody, anti-HIST1H4J antibody, anti-H4/E antibody, anti-H4FE antibody, anti-HI antibody

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Immunofluorescence analysis of human-liver tissue. 1, Histone H4 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

Immunofluorescence analysis of rat-heart tissue. 1, Histone H4 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

Western Blot analysis of various cells using primary antibody diluted at 1:1000.

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, Histone H4 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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