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HLA-DPB1, HLA-DQA1 Antibody

SKU: orb1252224

Description

HLA-DPB1, HLA-DQA1 Antibody

Images & Validation

Tested ApplicationsIF, IHC-P, WB
ReactivityHuman
Application Notes
WB: 1-2 ug/mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RTOptimal dilution of the HLA-DP/DQ/DR antibody should be determined by the researcher.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeIgG1, kappa
Clone No.CR3/43
ImmunogenHuman tonsil cells were used as the immunogen for the HLA-DP/DQ/DR antibody.
TargetHLA-DPB1, HLA-DQA1
PurificationProtein G affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Concentration0.2 mg/mL
DisclaimerFor research use only

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HLA-DPB1, HLA-DQA1 Antibody

IHC testing of FFPE human tonsil tissue with HLA-DP/DQ/DR antibody (clone CR3/43). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

HLA-DPB1, HLA-DQA1 Antibody

IHC testing of FFPE human tonsil tissue with HLA-DP/DQ/DR antibody (clone CR3/43). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

HLA-DPB1, HLA-DQA1 Antibody

Western blot testing of human Raji cell lysate with HLA-DP/DQ/DR antibody (clone CR3/43). Expected molecular weight ~36 kDa (alpha chain) and ~27 kDa (beta chain).

HLA-DPB1, HLA-DQA1 Antibody

SDS-PAGE Analysis of Purified, BSA-Free HLA-DP/DQ/DR Antibody (clone CR3/43). Confirmation of Integrity and Purity of the Antibody.

HLA-DPB1, HLA-DQA1 Antibody

IF/ICC staining of human Raji cells with HLA-DP/DQ/DR antibody (green) and Reddot nuclear stain (red).

HLA-DPB1, HLA-DQA1 Antibody

Flow cytometry testing of human Raji cells with HLA-DP/DQ/DR antibody at 1 ug/10^6 cells (blocked with goat sera); Red = isotype control, Blue = HLA-DP/DQ/DR antibody.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IF
Immunofluorescence
View Protocol

HLA-DPB1, HLA-DQA1 Antibody (orb1252224)

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