Hsd11b1 Antibody

SKU: orb1248849

This product can be custom made

KO/KD
Validated

Description

Hsd11b1 Antibody

Research Area

Metabolism Research

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, KO/KD Validated, WB
Reactivity	Mouse
Predicted Reactivity	Rat
Application Notes	Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 32kDa band observed in Mouse Lung lysates (calculated MW of 32.4kDa according to NP_032314.2). Recommended concentration: 0.3-1µg/mL. An additional band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunizing peptide. However, it remained visible in a KO background. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Goat
Clonality	Polyclonal
Immunogen	The immunogen for this antibody is: C-TARSEEGLQKVVSR
Target	Hsd11b1
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Hsd11b1, hydroxysteroid (11-beta) dehydrogenase 1, 11-DH, 11-beta-HSD1, HDL, HSD11, HSD11B, HSD11L, 11-beta-hydroxysteroid dehydrogenase 1, corticosteroid 11-beta-dehydrogenase isozyme 1, short chain dehydrogenase/reductase family 26C, member 1

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orb1248849 (0.3 ug/ml) staining of Mouse Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248849 (0.3 ug/ml) staining of Mouse Lung lysate (60ug protein in RIPA buffer). First lane shows wildtype and second lane shows knockout background. Primary incubation was overnight. Detected by fluorescence with Li-cor.

Quick Database Links

Gene Symbol

Hsd11b1

UniProt

P50172

RefSeq (Protein):NP_032314.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_032314.2

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Datasheet

Product Information

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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