HSP27 inhibitor J2

SKU: orb1299957

Description

J2 is a small molecule inhibitor of heat shock protein 27 (HSP27). It disrupts the formation of large HSP27 polymers, thereby blocking its chaperone function and compromising cellular stress protection. This inhibitor is a valuable research tool for studying HSP27's role in cancer and neurodegenerative disease models, both in vitro and in vivo.

Research Area

Metabolism Research, Signal Transduction

Images & Validation

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Key Properties

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CAS Number	2133499-85-9
MW	264.3
Purity	99.51% (May vary between batches)
Formula	C₁₃H₁₂O₄S
SMILES	Cc1cc(=O)c2c(O)cc(OCC3CS3)cc2o1
Target	HSP
Solubility	DMSO:20.83 mg/mL (78.81 mM);10% DMSO+90% Corn Oil:2 mg/mL (7.57 mM)

Bioactivity

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In Vivo

HSP27 inhibitor (J2) on radiation-induced lung inflammation in comparison to amifostine.In gross and histological findings,J2 treatment significantly inhibited immune cell infiltration in lung tissue, revealing anti-inflammatory potential of J2.Normal lung volume, evaluated by micro-CT analysis, in J2-treated mice was higher compared to that in irradiated mice.J2-treated mice reversed radiation-induced respiratory distress.However, amifostine did not show significant radioprotective effects in comparison to that of J2.In HSP27 transgenic mice, we observed increased immune cells recruitment and decreased volume of normal lung compared to wild type mice.Increased ROS production and oxidative stress after IR were down-regulated by J2 treatment, demonstrating antioxidant property of J2.J2 may be an effective therapeutic agent for radiation-induced lung injury.

Animal Research

Male C57BL/6 mice (age, 6 weeks; weight, 20–25 g), and were acclimatized (n = 5 per cage) for a week before irradiation. A single dose of 75 Gy was delivered to the left lung in a single fraction using image-guided small-animal irradiator (X-RAD 320) that was equipped with a collimator system composed of 3.5-cm-thick copper to produce focal radiation beams, as well as an imaging subsystem consisting of a fluorescent screen coupled to a charge-coupled-device camera. We selected 3-mm collimators to mimic clinical SBRT conditions by irradiating only a small volume of tissue. The mice were divided into six groups (n = 6–8 per group) as follows: (1) control (C);(2) irradiation (IR) - mice were exposed to a single dose of 75 Gy delivered to the left lung in a single fraction; (3) irradiation + J2 (IR + J2) 15 mg/kg of J2 was intraperitoneal administered on every other day after irradiation;(4) irradiation + amifostine (IR + Ami) 100 mg/kg of amifostine were intraperitoneal administered on every other day after irradiation; (5) J2 only (J2) 15 mg/kg of J2 were intraperitoneal administered on every other day without irradiation; (6) Amifostine only (Ami) 100 mg/kg of amifostine were intraperitoneal administered on every other day without irradiation. On day 14, mice were sacrificed by CO2 asphyxiation, and their lung tissues were collected for analysis.

Storage & Handling

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Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Inhibitor, HSP27 inhibitor J2, HSP-27 inhibitor J2, HSP27, Heat shock proteins, HSP, J2, J-2, J 2, inhibit

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Compound Identifiers

PubChem CID

135384973 ↗

Key Properties

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No computed properties available.

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