Human Extracellular Signal Regulated Kinase 2 (ERK2) ELISA Kit

SKU: orb775801

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Extracellular Signal Regulated Kinase 2(ERK2).

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 3000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ERK2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ERK2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ERK2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ERK2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	46.88-3000 pg/mL
Sensitivity	18.08 pg/mL
Concentration	3000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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MAPK1 ELISA Kit, P38 ELISA Kit, P40 ELISA Kit, ERK2 ELISA Kit, ERT1 ELISA Kit, MAPK2 ELISA Kit, P42MAPK ELISA Kit, P42-MAPK ELISA Kit, PRKM1 ELISA Kit, PRKM2 ELISA Kit, P41 ELISA Kit, P41mapk ELISA Kit, P41-mapk ELISA Kit, Mitogen-Activated Protein Kinase 1 ELISA Kit, Mitogen-activated protein kinase 2 ELISA Kit

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Human Extracellular Signal Regulated Kinase 2 (ERK2) ELISA Kit

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UniProt

P28482

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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