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Human IFN-beta
SKU: orb3002500
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Activity | ELISA: Immobilized Recombinant Human IFNAR2 (C-Fc)(Cat#orb2994011) at 2μg/ml (100 μl/well) can bind Recombinant Human IFNB1*. *: Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Human IFNB1 is 4.36ng/ml.(Regularly tested). |
| Tag | N-Fc |
| Molecular Weight | Predicted: 46.2kDa. Observed: 47-57kDa, reducing conditions |
| Expression Region | Met22-Asn187 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | ＜0.01EU/μg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM NaAC-HAC, 10%Terhalose, 50mM NaCl, 3%Mannitol, 0.01%Tween20, pH 5.5. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Interferon beta; IFN-beta; Fibroblast interferon; IFNB1; IFB; IFNB
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Human IFN-beta (orb3002500)
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