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Human IgG (H&L) Antibody Alkaline Phosphatase Conjugated
SKU: orb347232
Description
Images & Validation
−Item 1 of 2
| Tested Applications | DOT, ELISA, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:4,500, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Human IgG whole molecule |
| Purity | Anti-Human IgG (H&L) was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Human IgG and Human Serum. |
| Conjugation | AP |
Storage & Handling
−| Storage | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.1% (w/v) Sodium Azide |
| Concentration | 1.0 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−goat anti-Human IgG Alkaline Phosphatase Conjugation, goat anti-Human IgG ALP conjugate, goat anti-human IgG Alk Phos conjugated antibody
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Dot Blot of Alkaline Phosphatase conjugated Goat anti-Human IgG antibody. Antigen: Human IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.4 ng Lane 5 - 2.5 ng. Primary antibody: none. Secondary antibody: Alkaline Phosphatase conjugated Goat anti-Human IgG antibody at 1:1000 for 60 min at RT. Block: orb348637 for 60 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.
Western Blot of Alkaline Phosphatase conjugated Goat anti-Human IgG antibody. Lane 1: Human IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase Human secondary antibody at 1:1000 o/n at 4°C. Block: orb348637 for 30 min at RT. Reaction visualized using alkaline phosphatase for 30 seconds at RT. Predicted/Observed size: 50kDa, 25kDa for Human IgG.
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WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
DOT
Dot Blot
Human IgG (H&L) Antibody Alkaline Phosphatase Conjugated (orb347232)
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