Human STAT6 protein

SKU: orb392524

Description

Recombinant human STAT6 protein is produced by our E. coli expression system and the target gene encoding Ser627-Ser837 is expressed with a 6His tag at the C-terminus.

Research Area

Epigenetics

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	E. coli
Reactivity	Human
Protein Sequence	MSHYKPEQMG KDGRGYVPAT IKMTVERDQP LPTPELQMPT MVPSYDLGMA PDSSMSMQLG PDMVPQVYPP HSHSIPPYQG LSPEESVNVL SAFQEPHLQM PPSLGQMSLP FDQPHPQGLL PCQPQEHAVS SPDPLLCSDV TMVEDSCLSQ PVTAFPQGTW IGEDIFPPLL PPTEQDLTKL LLEGQGESGG GSLGAQPLLQ PSHYGQSGIS MSLEHHHHHH
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

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Storage	Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/Appearance	Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Signal transducer and activator of transcription 6; IL-4 Stat

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Quick Database Links

UniProt

P42226

NCBI Gene ID

6778

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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