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Human TGF-beta 1 Protein
SKU: orb257996
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Description
Images & Validation
−Item 1 of 4
| Application Notes |
|---|
Key Properties
−| Expression System | HEK293 |
|---|---|
| Target | TGF-beta 1 |
| Reactivity | Human |
| Tag | Native |
| Molecular Weight | 12.8 kDa (monomer) |
| Expression Region | Ala 279 - Ser 390 |
| Purity | 95% |
| Endotoxins | Less than 0.1 EU per μg by the LAL method / rFC method |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 12 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
|---|---|
| Form/Appearance | Powder |
| Buffer/Preservatives | Lyophilized from 0.22 μm filtered solution in 0.085% TFA in 30% ACN with trehalose as protectant |
| Disclaimer | For research use only |
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Immobilized Human TGFB1, premium grade (Cat. No. orb257996) at 0.2 μg/mL (100 μL/well) can bind Human TGFBR2, Fc Tag (Cat. No. orb257866) with a linear range of 0.2-2.5 ng/mL (QC tested).
Human TGF-beta 1 Protein (Cat. No. orb257996) immobilized on CM5 Chip can bind Human TGF-beta RI Protein, Fc Tag (Cat. No. orb1786177) with an affinity constant of 99.2 nM as determined in a SPR assay (Biacore 8K)
Human TGF-beta 1 Protein (Cat. No. orb257996) inhibits the Human IL-4, premium grade (Cat. No. orb257598) dependent proliferation the TF-1 cells. The specific activity of Human TGF-Beta 1 Protein, Tag Free is > 8.00ⅹ10^6 IU/mg, which is calibrated against transforming growth factor β1 (NIBSC code: 89/514)
Human TGF-beta 1 Protein (Cat. No. orb257996) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Quick Database Links
Gene Symbol
TGF-beta 1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
Cirka, Heather et al. Active Traction Force Response to Long-Term Cyclic Stretch Is Dependent on Cell Pre-stress Biophys J, 110, 1845-1857 (2016)
Human TGF-beta 1 Protein (orb257996)
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