You have no items in your shopping cart.
Human TNF alpha
SKU: orb3002254
Description
Images & Validation
−
Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Activity | BLI: Loaded Human TNF alpha-His(Cat#orb3002254) on HIS1K Biosensor, can bind Human TNF RII-mFc(Cat#orb2994890) with an affinity constant of 1.83 nM as determined in BLI assay. (Regularly tested) . Cell Based Assay: Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 30-150 pg/ml. (QC verified) |
| Tag | N-6His |
| Molecular Weight | Predicted: 21.8 KDa. Observed: 18 KDa, reducing conditions |
| Expression Region | Gly57-Leu233 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM Histidine, 8 %Trehalose, 0.05%Tween80, pH 5.0. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; TNF; TNFA; TNFSF2
Similar Products
−- Item 1 of 6
- Item 1 of 1
Human Tumor Necrosis Factor Alpha (TNFa) ELISA Kit [orb775055]
Human
15.63-1000 pg/mL
6.4 pg/mL
96 T, 48 T - Item 1 of 3
Human TNF-alpha Protein [orb257883]
ELISA, SDS-PAGE
Unconjugated
>95% as determined by SDS-PAGE and SEC-MALS.
17.4 kDa
1 mg, 100 μg, 20 μg - Item 1 of 6
DOCK180/DOCK1 Rabbit Polyclonal Antibody [orb1291684]
ELISA, FC, IF, IHC, WB
Human
Rabbit
Polyclonal
Unconjugated
100 μg - Item 1 of 6
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
Quick Database Links
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
Human TNF alpha (orb3002254)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Usually dispatched within 5-10 working days