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Description
Research Area
Immunology & Inflammation
Images & Validation
−
Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Activity | BLI: Loaded Human TNF alpha-His(Cat#orb3002254) on HIS1K Biosensor, can bind Human TNF RII-mFc(Cat#orb2994890) with an affinity constant of 1.83 nM as determined in BLI assay. (Regularly tested) . Cell Based Assay: Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 30-150 pg/ml. (QC verified) |
| Tag | N-6His |
| Expression Region | Gly57-Leu233 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| MW | Predicted: 21.8 KDa. Observed: 18 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM Histidine, 8 %Trehalose, 0.05%Tween80, pH 5.0. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; TNF; TNFA; TNFSF2
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Recombinant Human TNF alpha Protein (orb3002254)
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