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Human UBE2B protein

SKU: orb392653

Description

Recombinant human UBE2B protein is produced by our E. coli expression system and the target gene encoding Met1-Ser152 is expressed.

Research Area

Epigenetics & Chromatin

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceE. coli
TargetUBE2B
ReactivityHuman
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
PurityGreater than 95% as determined by reducing SDS-PAGE.
Protein SequenceGSHMSTPARR RLMRDFKRLQ EDPPVGVSGA PSENNIMQWN AVIFGPEGTP FEDGTFKLVI EFSEEYPNKP PTVRFLSKMF HPNVYADGSI CLDILQNRWS PTYDVSSILT SIQSLLDEPN PNSPANSQAA QLYQENKREY EKRVSAIVEQ SWNDS

Storage & Handling

StorageStore it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.2 μM filtered solution of 10mM HEPES, pH 7.5.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

RAD6B; Ubiquitin-conjugating enzyme E2 B; RAD6 homolog B; HR6B; hHR6B; Ubiquitin carrier protein B; Ubiquitin-conjugating enzyme E2-17 kDa; Ubiquitin-protein ligase B

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Available Sizes

Select a size below

10 μg
$ 300.00
50 μg
$ 680.00
500 μg
$ 3,230.00
DispatchUsually dispatched within 5-10 working days
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