VEGFA Rabbit Monoclonal Antibody

SKU: orb2960282

Description

Anti-Human VEGFA Antibody (BUCC2753) is a rabbit monoclonal antibody detecting Vascular endothelial growth factor A in ELISA, FCM. Suitable for Human. Highlights Rabbit Monoclonal: High specificity, affinity, and excellent lot-to-lot consistency. Flow Cytometry: Validated for flow cytometry applications.

Research Area

Cardiovascular Research; Cell Biology

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, FC
Reactivity	Human

Key Properties

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Antibody Type	Recombinant Antibody
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone No.	BUCC2753
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin	Please contact with the lab for this information.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M PBS, pH 7.4.
Concentration	Lot dependent
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Vascular endothelial growth factor A, VPF, VEGFA, VEGF, Vascular permeability factor, VEGF-A

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Flow-cytometry using anti-human VEGFA antibody. HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human VEGFA antibody monoclonal antibody (Catalog # orb2960282, Green Histogram) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rabbit antibody and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human VEGFA antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human VEGFA antibody monoclonal antibody (Catalog # orb2960282, Green Histogram) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rabbit antibody and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human VEGFA antibody. VEGFA Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human VEGFA antibody monoclonal antibody (Catalog # orb2960282, Green Histogram) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rabbit antibody and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Anti-Human VEGFA Antibody.

Quick Database Links

UniProt

P15692

UniProt Details

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No UniProt data available

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Protocol Information

Flow Cytometry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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