VEGFA Mouse Monoclonal Antibody

SKU: orb2959170

Description

Anti-Rat VEGFA Antibody (BD42X6) is a mouse monoclonal antibody detecting Vascular endothelial growth factor A in Blocking, ELISA. Suitable for Human, Mouse, Rat, and Rabbit. Highlights Multi-Species: Cross-reactive for translational research.

Research Area

Cardiovascular Research; Cell Biology

Images & Validation

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Item 1 of 4

Tested Applications	Blocking, ELISA
Reactivity	Human, Mouse, Rabbit, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG2a, kappa
Clone No.	BD42X6
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin	Please contact with the lab for this information.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M PBS, pH 7.4.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Vascular endothelial growth factor A, VPF, VEGFA, VEGF, Vascular permeability factor, VEGF-A

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Flow-cytometry using FITC anti-human VEGF antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human VEGF monoclonal antibody (Catalog: orb2959170, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using PE anti-human VEGF antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human VEGF monoclonal antibody (Catalog: orb2959170, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Anti-Rat VEGFA Antibody.

Western blot analysis was performed using anti-VEGFA/VEGF165 monoclonal antibody at 1 µg/mL on various samples. Lane 1: recombinant human VEGFA/VEGF165 (Catalog No: orb2969097), Lane 2: negative control.

Quick Database Links

UniProt

P16612

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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