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IL-2 Antibody

SKU: orb345092

Description

IL-2 antibody

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:20,000, WB: 1:500-1:2,000
ReactivityFeline
Application Notes
This protein A purified IL-2 antibody has been tested by ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15.4 kDa in size corresponding to feline IL-2 by western blotting in the appropriate cell lysate or extract.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenThis protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast corresponding to 134 amino acids of mature cat IL-2.
PurityThis product was protein A purified from monospecific antiserum by chromatography. This antibody is specific for cat IL-2 protein. A BLAST analysis was used to suggest cross-reactivity with IL-2 from cat based on 100% homology; cross-reactivity with Northern elephant seal and gray seal based on 90% homology with the immunizing sequence. Cross-reactivity with IL-2 from other sources has not been determined.
ConjugationUnconjugated

Storage & Handling

StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLyophilized
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration1.0 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

rabbit anti-Interleukin-2, rabbit anti-IL-2 antibody, IL2, T-cell growth factor, TCGF, Aldesleukin, Lymphokine

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IL-2 Antibody

Western blot using Biorbyt's protein-A purified anti-Cat IL-2 antibody shows detection of recombinant Cat IL-2 at 15.4 kDa, raised in yeast. Primary antibody was diluted to 1 µg/ml. 3% BSA from Biorbyt's Bovine Serum Albumin Solution was used for blocking. Secondary antibody (Goat anti-Rabbit IgG IRDye® 800) was used at 1:20000.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

IL-2 Antibody (orb345092)

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Available Sizes

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100 μg
$ 890.00
DispatchUsually dispatched within 5-10 working days
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