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IL-2 Antibody
SKU: orb345093
Description
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | ELISA: 1:20,000, WB: 1:500-1:2,000 |
| Reactivity | Feline |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast corresponding to 134 amino acids of mature cat IL-2. |
| Purity | This product was protein A purified from monospecific antiserum by chromatography. This antibody is specific for cat IL-2 protein. A BLAST analysis was used to suggest cross-reactivity with IL-2 from cat based on 100% homology; cross-reactivity with Northern elephant seal and gray seal based on 90% homology with the immunizing sequence. Cross-reactivity with IL-2 from other sources has not been determined. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.15 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-Interleukin-2 antibody, rabbit anti-IL-2 antibody, IL2, T-cell growth factor, TCGF, Aldesleukin, Lymphokine
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Western blot using Biorbyt's protein-A purified anti-Cat IL-2 antibody shows detection of recombinant Cat IL-2 at 15.4 kDa, raised in yeast. Primary antibody was diluted to 1 µg/ml. 3% BSA from Biorbyt's Bovine Serum Albumin Solution was used for blocking. Secondary antibody (Goat anti-Rabbit IgG IRDye® 800) was used at 1:20000.
Quick Database Links
UniProt
RefSeq (Protein):NP_001036802.1
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001036802.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IL-2 Antibody (orb345093)
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