Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human IL-2R gamma (51-150/369aa)
Target	IL2RG
Molecular Weight	41 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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IL2 Receptor gamma; IL2R gamma; IL 2R gamma; IL-2 receptor gamma precursor; IL2 Receptor gamma; CD132; CD 132; common cytokine receptor gamma chain; gamma C; gamma(c); IL-2R gamma chain; IL2RG; IMD4; interleukin 2 receptor, gamma; P64; SCIDX; SCIDX1; Cytokine receptor common subunit gamma; gamma C; gammaC; IL-2 receptor subunit gamma; IL-2R subunit gamma; IL-2RG; IL2RG_HUMAN; Interleukin-2 receptor subunit gamma; p64; SCIDX; SCIDX1.

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Black line: Positive blank control (U937), Negative blank control (MCF7), Green line: Primary Antibody (Rabbit Anti-2R gamma antibody (orb13311)), Orange line: Isotype Control Antibody (Rabbit IgG). Blue line: Secondary Antibody (Goat anti-rabbit IgG-AF647), U937 (Positive) and MCF7 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with 2R gamma Antibody (orb13311) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody (blue) incubation for 40 min at room temperature. Acquisitions of 20000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Blank control: U937 (blue). Primary Antibody: Rabbit Anti-IL-2R, gamma antibody (orb13311), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb13311, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

SHSY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (IL-2R gamma) polyclonal Antibody, Unconjugated (orb13311) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

IL2RG

UniProt

P31785

UniProt Details

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No UniProt data available

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