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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:300 - 1:1,000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with mature length recombinant human IL-6 produced in E.coli. |
| Target | IL6 |
| Purity | IL-6 antibody detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. The antiserum is also useful for neutralization of human of IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity. This product has minimal reactivity with mouse IL-6. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: None. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−rabbit Anti-IL-6 antibody, rabbit anti-interleukin-6 antibody, HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL 6, IL6 protein, Interferon beta 2, Interleukin 6, BSF2, CDF
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Immunohistochemistry of Rabbit Anti-IL6-Antibody. Tissue: human lymph nodes at pH9. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-IL-6 antibody at 10 µg/ml for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Localization: IL-6 is cytoplasmic. Staining: IL-6 as precipitated brown signal (B 20x, B 40x) hematoxylin purple nuclear counterstain. Negative Control (A 20x).
Western blot using Biorbyt's anti-Human IL6 antibody shows detection of a band ~24 kDa in size corresponding to recombinant human IL6 (arrowhead). Molecular weight markers are also shown. After transfer, the membrane was blocked overnight with 1% BSA in TTBS followed by reaction with primary antibody at a 1:500 dilution. Detection occurred using HRP conjugated anti-Rabbit IgG (p/n orb347654) secondary antibody diluted 1:40000 in blocking buffer (p/n orb348637) and FemtoMax™ Chemiluminescent substrate.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000591.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IL6 Antibody (orb345128)
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