IL6 Antibody

Western Blot of Rabbit Anti-IL-6 Antibody. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: Human Rec. IL-6/HeLa Whole Cell Lysate [0.05 µg/10 µg]. Lane 3: Human Rec. IL-6/HeLa Whole Cell Lysate [0.02 µg/10 µg]. Lane 4: Human Rec. IL-6/HeLa Whole Cell Lysate [0.01 µg/10 µg]. Lane 5: HeLa Whole Cell Lysate (p/n orb348668) [10 µg]. Primary Antibody: Anti-IL-6 at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP conjugate (p/n orb347654) at 1:70000 for 30 mins at RT. Block: BlockOut Buffer (p/n orb348644). Predicted MW: ~23kDa. Observed MW: ~19 Exposure: 2 seconds.

Western blot using Biorbyt's Anti-IL6 antibody. Protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. The blot shows detection of a band ~21 kDa in size corresponding to anti-IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST. Detection occurred using peroxidase conjugated Goat anti-Rabbit IgG (p/n orb347654) secondary antibody diluted 1:40000 in blocking buffer (p/n orb348637) for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate.