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IL-6 Recombinant Protein
Description
Research Area
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Recombinant IL-6 is fully biologically active when compared to standards.ED50 is less than 0.1 ng/ml as determined by the dose-dependent stimulation of human TF-1 cells.Specific Activity of 5.0 x 107 IU/mg. |
| Target | IL6 |
| Tag | Tag Free |
| Molecular Weight | 20.9 kD |
| Protein Sequence | Pro29-Met212 |
| Purity | Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Lyophilized from a 0.2 um filtered solution of 20mM HAc-NaAc, 150mM NaCl, pH 5.0. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Recombinant Human Interleukin-6 (IL6) Protein (Active) [orb1881858]
Greater than 95% as determined by SDS-PAGE
22.8 kDa
Yeast
1 mg, 100 μg, 20 μgRecombinant Rhesus Macaque IL-6 Protein [orb2992743]
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 21.8 KDa. Observed: 22-33 KDa, reducing conditions
1 mg, 500 μg, 50 μg, 10 μgRecombinant Mouse IL-6 Protein [orb2994610]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (Regularly tested)
Predicted: 21.8 KDa. Observed: 22 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human IL-6 Protein [orb2995250]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 20.9 KDa. Observed: 20 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg

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IL-6 Recombinant Protein (orb1229854)
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