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IL-6 Recombinant Protein
Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Target | Il6 |
| Tag | His Tag |
| Molecular Weight | 23.6 kDa |
| Protein Sequence | Phe 25 - Thr 211 |
| Purity | >90% as determined by SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Recombinant Human Interleukin-6 (IL6) Protein (Active) [orb1881858]
Greater than 95% as determined by SDS-PAGE
22.8 kDa
Yeast
1 mg, 100 μg, 20 μgRecombinant Rhesus Macaque IL-6 Protein [orb2992743]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 21.8 KDa. Observed: 22-33 KDa, reducing conditions
1 mg, 500 μg, 50 μg, 10 μgRecombinant Mouse IL-6 Protein [orb2994610]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (Regularly tested)
Predicted: 21.8 KDa. Observed: 22 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human IL-6 Protein [orb2995250]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 20.9 KDa. Observed: 20 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg

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Mouse IL-6, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Immobilized Mouse Human IL-6, His Tag at 5 ug/ml (100 uL/well) can bind Biotinylated Mouse IL-6 R alpha, His, Avitag with a linear range of 20-156 ng/mL.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_112445.1 |
|---|
Documents Download
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Protocol Information
IL-6 Recombinant Protein (orb1231525)
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