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IRF3 Recombinant Rabbit Monoclonal Antibody
SKU: orb500087
Description
Images & Validation
−Item 1 of 10
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:1000-5000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | 4C3 |
| Immunogen | A synthesized peptide derived from human IRF3 (82-123aa) |
| Target | IRF3 |
| Molecular Weight | 47 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/1ml |
| Disclaimer | For research use only |
Alternative Names
−Interferon regulatory factor 3; IRF-3; IRF 3; IRF3_HUMAN.
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25 ug total protein per lane of various lysates probed with IRF3 monoclonal antibody, unconjugated (orb500087) at 1:3000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with IRF3 Monoclonal Antibody, Unconjugated (orb500087) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with IRF3 Monoclonal Antibody, Unconjugated (orb500087) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with IRF3 Monoclonal Antibody, Unconjugated (orb500087) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Heart, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with IRF3 Monoclonal Antibody, Unconjugated (orb500087) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with IRF3 Monoclonal Antibody, Unconjugated (orb500087) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with IRF3 Monoclonal Antibody, Unconjugated (orb500087) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with IRF3 Monoclonal Antibody, Unconjugated (orb500087) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with IRF3 Monoclonal Antibody, Unconjugated (orb500087) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with IRF3 Monoclonal Antibody, Unconjugated (orb500087) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Quick Database Links
Gene Symbol
IRF3
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
IRF3 Recombinant Rabbit Monoclonal Antibody (orb500087)
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