JG-98

SKU: orb1226234

Description

JG-98 is an allosteric Hsp70 inhibitor, displays >3-fold more active, greater stability than MKT-077 against the breast cancer cell lines MDA-MB-231 and MCF-7 (EC50 of 0.4 uM and 0.7 uM, respectively); strongly affects autophagic flux, increases p62 oligomerization and reduces p62 monomer; destabilizes FoxM1 and relieved suppression of downstream effectors, including p21 and p27.

Images & Validation

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Key Properties

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CAS Number	1456551-16-8
MW	534.532
Purity	>98% (HPLC)
Formula	C₂₄H₂₁Cl₂N₃OS₃
SMILES	CCN1C(=CC2=[N+](C=CS2)CC3=CC=CC=C3)SC(=C4N(C5=C(S4)C=C(C=C5)Cl)C)C1=O.[Cl-]
Target	HSP
Solubility	DMSO: Soluble （ < 1 mg/ml refers to the product slightly soluble or insoluble )

Bioactivity

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In Vivo

JG-98 (3 mg/kg; i.p.; on days 0, 2, and 4) suppresses tumor growth in xenograft models bearing MCF7 and HeLa cells. Animal model: 6-week-old NCR mice (xenografts of MCF7 and HeLa cells). Dosage: 3 mg/kg. Administration: Intraperitoneal injection; on days 0, 2, and 4. Result: Limited tumor growth, but somewhat less effectively.

In Vitro

JG-98 (30 nM-30 μM; 72 hours) has antiproliferative activity across a range of cell lines with the EC50s between ~0.3 and 4 μM. JG-98 (10 μM; 48 hours) activates apoptotic mediators (cleavage of caspase-3 and PARP) in MDA-MB-231 cells. JG-98 destabilizes FoxM1 and relieves suppression of downstream effectors, including p21 and p27. Cell Proliferation Assay Cell line: MCF-7, MDA-MB-231, A375, MeWo, HeLa, HT-29, SKOV3, Jurkat, mouse embryonic fibroblasts (MEF), MM1.R, INA6, RPMI-8226, JJN-3, U266, NCI-H929, L363, MM1.S, KMS11, LP-1, AMO-1, OPM1, OPM2 cells Concentration: 30 nM-30 μM. Incubation time: 72 hours. Result: Active against all of the lines tested, and the EC50s were variable (between ~0.3 μM and 4 μM). Normal MEFs and OPM1 and OPM2 were relatively less sensitive. Western blot analysis. Cell line: MDA-MB-231 cells. Concentration: 10 μM. Incubation time: 48 hours. Result: Incudes apoptotic mediators cleavage of caspase 3 and PARP.

Storage & Handling

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Storage	Storage temperature: -20°C. Stability: ≥ 2 years
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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JG98

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HSP

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JG-98 (orb1226234)

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Purity:	99.13%
CAS Number:	1456551-16-8
MW:	534.53
Formula:	C₂₄H₂₁Cl₂N₃OS₃

JG-98

Description

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

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JG-98 (orb1226234)

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