JG-98

SKU: orb1301863

Description

JG-98

Research Area

Cell Biology, Metabolism Research, Signal Transduction

Images & Validation

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Key Properties

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CAS Number	1456551-16-8
MW	534.53
Purity	99.13%
Formula	C₂₄H₂₁Cl₂N₃OS₃
SMILES	[Cl-].CCn1\c(=C\c2scc[n+]2Cc2ccccc2)s\c(=C2\Sc3cc(Cl)ccc3N2C)c1=O
Target	Apoptosis,HSP
Solubility	DMSO:5 mg/mL (9.35 mM)

Bioactivity

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Target IC50	HSP70 (MDA-MB-231):0.4μM\|HSP70(MCF-7):0.7 μM
In Vivo	JG-98 (3 mg/kg) suppressed tumor growth in a HeLa xenograft model, though somewhat less effective .
In Vitro	JG-98 had a potency of 0.4 ± 0.03 μM against MDA-MB-231 cells and an EC50 value of 0.7 ± 0.2 μM for MCF7 cells. In MDA-MB-231 cells, the treatment of JG-98 activated apoptotic mediators (caspase-3 and PARP). Treatment of both MDA-MB-231 and MCF7 cells with JG-98 strongly affected autophagic flux . JG-98 has antiproliferative activity (EC50 values between 0.3 and 4 μmol/L) across cancer cell lines from multiple origins. JG-98 destabilized FoxM1 and relieved suppression of downstream effectors, including p21 and p27 . Read more...
Cell Research	Cell viability was determined using an MTT colorimetric assay with the following modifications. Briefly, cells (5 x 10^3 ) were plated into 96-well assay plates in 0.1 ml media and allowed to attach overnight. Cells were then treated with compound at various concentrations in 0.2 mL media. After the 72-hour incubation period, cells were washed in PBS (3 x 100 μL), and 10 μL MTT reagent was added with 100 μL fresh media. Cells were then incubated for 4 hr in a humidified chamber at 37 oC with 5% CO2. Insoluble formazan crystals were solubilized by addition of 0.1 mL detergent solution (4 hr at room temp., dark). Resulting colored solutions were then quantified at an absorbance of 570 nm . Read more...
Animal Research	Briefly, one million MCF7 or HeLa cells in Matrigel were subcutaneously injected bilaterally into 6 week old NCR mice. Once tumors were established, JG-98 (3 mg/kg; n=5) or vehicle control (1:1 PBS: DMSO; n=5) was introduced interperitoneally on days 2, 4 and 6. Tumor growth (10 tumors/5 mice) was measured by caliper every other day . Read more...

Storage & Handling

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Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Apoptosis, Heat shock proteins, Inhibitor, HSP, HSP70, inhibit, JG98, JG-98, JG 98

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Compound Identifiers

PubChem CID

72547053 ↗

Key Properties

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No computed properties available.

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JG-98 (orb1301863)

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Purity:	>98% (HPLC)
CAS Number:	1456551-16-8
MW:	534.532
Formula:	C₂₄H₂₁Cl₂N₃OS₃

JG-98

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

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