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Lag3 Antibody
SKU: orb1671700
Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 2
| Tested Applications | Blocking, FC, IP |
|---|---|
| Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Recombinant |
| Isotype | IgG kappa |
| Clone No. | C9B7W |
| Immunogen | This antibody was raised by immunising Lewis rats with a murine LAG-3:Ig fusion protein. |
| Target | Lag3 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD223, LAG-3, Lymphocyte activation gene 3 protein
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Immunofluorescence staining of mouse splenocytes using anti-Lag3 antibody (C9B7W). Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of C9B7W (orb1671700) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671700- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.
Binding curve of anti-Lag3 antibody C9B7W (orb1671700) to recombinant mouse Lag3 Fc-Fusion Protein. ELISA Plate coated with recombinant mouse Lag3 Fc-Fusion Protein (Pr00162-1.9; Antibody) at a concentration of 2 ug/ml. A 4-fold serial dilution from 5-000 ng/ml was performed using orb1671700. For detection- a 1:4000 dilution of HRP-lnti-ribody was used.
Quick Database Links
Gene Symbol
Lag3
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
FC
Flow Cytometry
IP
Immunoprecipitation
Lag3 Antibody (orb1671700)
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