MCP1 Rabbit Polyclonal Antibody

SKU: orb13563

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Description

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, WB
Dilution range	WB=1:500-2000, ELISA=1:5000-10000
Reactivity	Mouse, Rat
Predicted Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human MCP-1 (24-99/99aa)
Target	CCL2
Molecular Weight	11 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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MCP-1/CCL2; C-C motif chemokine 2; CCL 2; CCL2; CCL2_HUMAN; Chemokine(C C motif) ligand 2; Chemokine C C motif ligand 2; Chemokine CC Motif Ligand 2; GDCF 2; GDCF 2 HC11; GDCF-2; GDCF2; HC11; HSMCR30; HSMCR30; JE; MCAF; MCP 1; MCP-1; MGC9434; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; SCYA2; Small inducible cytokine A2(monocyte chemotactic protein 1, homologous to mouse Sig je); Small inducible cytokine A2; Small inducible cytokine subfamily A(Cys Cys), member 2; Small inducible cytokine subfamily A Cys Cys member 2; Small-inducible cytokine A2; SMC CF; SMC-CF; SMCCF.

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Sample: Lane 1: Lung (Mouse) Lysate at 40 ug, Lane 2: Spleen (Mouse) Lysate at 40 ug, Lane 3: Lymph node (Mouse) Lysate at 40 ug, Lane 4: Lung (Rat) Lysate at 40 ug, Lane 5: Spleen (Rat) Lysate at 40 ug, Lane 6: Lymph node (Rat) Lysate at 40 ug, Primary: Anti-MCP1 (orb13563) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 11 kD, Observed band size: 11 kD.

Quick Database Links

Gene Symbol

CCL2

UniProt

P13500

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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IHC (3)

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Mouse (3)

Longobardi, L. et al. Role of the C-C chemokine receptor-2 in a murine model of injury-induced osteoarthritis Osteoarthritis Cartilage, 25, 914-925 (2017)

PubMed: 27856294

Applications

IHC

Reactivity

Mouse

Spencer, A. T. et al. Behaviour of intracerebral autografts of mouse tail skin pre-treated with a single application of 20-methylcholanthrene Nature, 207, 649-650 (1965)

PubMed: 5327560

Applications

IHC

Reactivity

Mouse

Ariel E. Timkovicha, G. Aaron Hollinga, Maryam F. Afzalia, John Kisidayb, and Kelly S. Santangelo TLR4 antagonism provides short-term but not long-term clinical benefit in a full-depth cartilage defect mouse model Connective Tissue Research, (2023)

PubMed: 37898909

Applications

IHC

Reactivity

Mouse