MEK1 Recombinant Rabbit Monoclonal Antibody

SKU: orb1817004

Description

Research Area

Cell Biology, MAPK/ERK Signalling Pathway, Tyrosine Kinases

Images & Validation

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Item 1 of 1

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Mouse

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	B5E1
Immunogen	A synthesized peptide derived from human MEK1 (1-40aa)
Target	Map2k1
Molecular Weight	43 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	PBS, Glycerol, rAlbumin.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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mitogen activated protein kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK1; Map2k1; MAPKK1; Mek1; MEKK1; Prkmk1; MP2K1_HUMAN.

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MEK1 Recombinant Rabbit Monoclonal Antibody

Sample: Lane 1: Mouse NIH/3T3 cell lysates, Lane 2: Mouse Muscle tissue lysates, Lane 3: Mouse Liver tissue lysates, Lane 4: Mouse Lung tissue lysates, Lane 5: Human HeLa cell lysates, Lane 6: Human MDA-MB-231 cell lysates, Lane 7: Human A431 cell lysates, Lane 8: Human Jurkat cell lysates, Primary: Anti-MEK1 (orb1817004) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kDa, Observed band size: 43 kDa.

Quick Database Links

Gene Symbol

Map2k1

UniProt

Q02750

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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