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MMP3 Antibody

SKU: orb2642538

Description

The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP3, MMP10 and MMP11 (also designated stromelysin-1, 2 and 3, respectively) activate procollagenase. MMP3 activation of procollagenase can occur via two pathways. Direct activation by MMP3 is slow and activation by MMP3 in conjunction with tissue or plasma proteinases is rapid. MMP10 is expressed in small intestine, and at lower levels in lung and heart. MMP11 is specifically expressed in stromal cells of breast carcinomas and contributes to epithelial cell malignancies.

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA (order BSA/sodium azide-free format for coating),Western blot: 1-2ug/ml
ReactivityHuman
Application Notes
Optimal dilution of the MMP3 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG2b, kappa
Clone No.MMP3/2655
ImmunogenA portion of amino acids 236-363 was used as the immunogen for the MMP3 antibody.
PurificationProtein G affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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MMP3 Antibody

Western blot testing of human Raji, HEK293, and HT-29 cell lysate with MMP3 antibody. Predicted molecular weight ~54 kDa.

MMP3 Antibody

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using MMP3 antibody. These results demonstrate the foremost specificity of the MMP3/2655 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

MMP3 Antibody (orb2642538)

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Available Sizes

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100 μg
$ 750.00
DispatchUsually dispatched within 5-10 working days
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