Mouse EBI3 protein

SKU: orb707238

Description

Recombinant Mouse IL-27 Protein is produced by mammalian expression system and the target gene encoding Tyr19-Pro228 & Phe29-Ser234 is expressed

Research Area

Epigenetics

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	Human cells
Reactivity	Mouse
Protein Sequence	YTETALVALS QPRVQCHASR YPVAVDCSWT PLQAPNSTRS TSFIATYRLG VATQQQSQPC LQRSPQASRC TIPDVHLFST VPYMLNVTAV HPGGASSSLL AFVAERIIKP DPPEGVRLRT AGQRLQVLWH PPASWPFPDI FSLKYRLRYR RRGASHFRQV GPIEATTFTL RNSKPHAKYC IQVSAQDLTD YGKPSDWSLP GQVESAPHKP GGGSGGGSGG GSGGGSFPTD PLSLQELRRE FTVSLYLARK LLSEVQGYVH SFAESRLPGV NLDLLPLGYH LPNVSLTFQA WHHLSDSERL CFLATTLRPF PAMLGGLGTQ GTWTSSEREQ LWAMRLDLRD LHRHLRFQVL AAGFKCSKEE EDKEEEEEEE EEEKKLPLGA LGGPNQVSSQ VSWPQLLYTY QLLHSLELVL SRAVRDLLLL SLPRRPGSAW DSVDHHHHHH
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.

Storage & Handling

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Storage	Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/Appearance	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Disclaimer	For research use only

Alternative Names

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IL27B; Interleukin-27 subunit beta; IL-27 subunit beta; IL-27B; Epstein-Barr virus-induced gene 3 protein; EBV-induced gene 3 protein; IL27A; IL30; Interleukin-27 subunit alpha; IL-27 subunit alpha; IL-27-A; IL27-A; Interleukin-30; p28

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Quick Database Links

UniProt

Q8K3I6, O35228

NCBI Gene ID

50498, 246779

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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