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Mouse IgG Fc Antibody
SKU: orb750706
Description
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | Antiserum |
| Immunogen | Mouse IgG F(c) fragment |
| Purity | This product was prepared from monospecific antiserum by delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab). |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 85 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−goat anti-Mouse IgG F(c) fragment Antibody, goat anti-Mouse IgG Fc fragment Antibody, rabbit anti Mouse IgG Fc
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KIF20B knockdown inhibits cell proliferation. (D) Expression of proliferation marker PCNA in shRNA cells and control cells, measured by western Blotting. Primary antibodies used mouse anti-(PCNA) proliferating cell nuclear antigen [1:1000] and mouse anti-β-actin [1:500] with goat anti-mouse Fc secondary antibody [1:10000]. Knocking down KIF20B inhibited PCNA expression. *P < 0.05 vs. control. KIF, kinesin family member; PCNA, proliferating cell nuclear antigen; shRNA, short hairpin RNA.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Mouse IgG Fc Antibody (orb750706)
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