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Mouse IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed
SKU: orb347406
Description
Images & Validation
−| Tested Applications | DOT, FC, IF, WB |
|---|---|
| Dilution Range | FC: 1:100 - 1:250, IF: 1:100 - 1:250 |
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in goat. |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, conjugation to phycoerythrin and subsequent purification, and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins. |
| Conjugation | RPE |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 0.5 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−goat anti-Mouse IgG phycoerythrin conjugated Antibody, Gt-a-Mouse IgG (H&L) Phycoerythrin conjugated, Mouse IgG(H&L) Phycoerythrin Antibody in goat, goat anti-Mouse IgG PE conjugated Secondary Antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
DOT
Dot Blot




