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Mouse IgG (H&L) Antibody Rhodamine Conjugated
SKU: orb347357
Description
Images & Validation
−Item 1 of 2
| Tested Applications | DOT, FC, FLISA, IF, WB |
|---|---|
| Dilution range | FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000 |
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Mouse IgG whole molecule |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum. |
| Conjugation | TRITC |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 2.0 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−goat anti-Mouse IgG rhodamine conjugated Antibody, goat anti-Mouse IgG Antibody TRITC conjugation
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Dot Blot of Rhodamine Conjugated Goat-a-Mouse IgG. Antigen: Mouse IgG. Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng Primary antibody: none Secondary antibody: Rhodamine Conjugated Goat-a-Mouse IgG secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 60 min at RT.
Western Blot of Rhodamine Conjugated Goat anti-Mouse IgG Secondary Antibody. Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Rhodamine goat secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): none.
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
DOT
Dot Blot
Mouse IgG (H&L) Antibody Rhodamine Conjugated (orb347357)
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