Mouse IgG (H&L) Antibody Rhodamine Conjugated

SKU: orb347494

Description

Mouse IgG (H&L) antibody (TRITC)

Images & Validation

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Item 1 of 2

Tested Applications	DOT, FC, FLISA, IF, WB
Dilution range	FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Reactivity	Mouse
Application Notes	Anti-Mouse IgG Rhodamine Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

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Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Mouse IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG, and Mouse Serum.
Conjugation	TRITC

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	2.0 mg/mL
Disclaimer	For research use only

Alternative Names

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Rabbit Anti-Mouse IgG Antibody rhodamine Conjugation, Rabbit Anti-Mouse IgG Antibody rhodamine Conjugated, Rabbit Anti-Mouse IgG TRITC Conjugated Antibody

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Mouse IgG (H&L) Antibody Rhodamine Conjugated

Fluorescence-activated cell sorting analysis after co-icubation of HCT116 cells with FITC-labeled CaIX-P1 and rhodamine-labeled anti-human CAIX mAb. Low left box: unlabeled cells (autofluorescence). Low right box: cells labeled only with FITC-CaIX-P1. Upper left box: cells labeled only with rhodamine-anti-CAIX-mAb. Upper right box: Cells labeled with both FITC-CaIX-P1 and rhodamine-anti-CAIX-mAb.

Western blot of Rhodamine conjugated Rabbit Anti-Mouse IgG secondary antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Rhodamine rabbit secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None.

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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Immunofluorescence

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DOT

Dot Blot

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