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NEK2 Antibody
SKU: orb1538570
Description
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, IHC, IHC-P, IP, WB |
|---|---|
| Dilution Range | ELISA, IHC, IHC-P (5 µg/ml), IP, WB |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG2a,k |
| Clone No. | 2F6 |
| Immunogen | NEK2 (AAH43502, 331 aa ~ 445 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Target | NEK2 |
| Purification | Purified from ascites by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS, pH 7.4 |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−NEK2, NIMA-related kinase 2, NEK2A, HsPK21, NimA-like protein kinase 1, NLK1, HSPK 21, NimA-related protein kinase 2
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Anti-NEK2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Western blot of NEK2 expression in transfected 293T cell line by NEK2 monoclonal antibody clone 2F6.
Detection limit for recombinant GST tagged NEK2 is approximately 0.3 ng/ml as a capture antibody.
Quick Database Links
Gene Symbol
NEK2
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IHC-P
Immunohistochemistry Paraffin
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
NEK2 Antibody (orb1538570)
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