Nephrin Rabbit Polyclonal Antibody

SKU: orb499749

Description

Research Area

Endothelial, Varscular System

Images & Validation

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Item 1 of 1

Tested Applications	WB
Dilution Range	WB=1:500-2000
Reactivity	Human, Mouse
Predicted Reactivity	Rat

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Nephrin (451-550/1241aa)
Target	NPHS1
Molecular Weight	138 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CNF; NPHN; Nephrosis 1 congenital Finnish type; NPHS 1; NPHS1; Renal glomerulus specific cell adhesion receptor; Renal glomerulus-specific cell adhesion receptor; NPHN_HUMAN

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Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-Nephrin (orb499749) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 138 kD, Observed band size: 170 kD.

Quick Database Links

Gene Symbol

NPHS1

UniProt

O60500

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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