NES Rabbit Polyclonal Antibody

SKU: orb1100522

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Description

anti- Nestin antibody

Research Area

Developmental Biology, Epigenetics

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IF, IHC, WB
Dilution Range	WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:20-1:200
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	nestin
Target	NES
Molecular Weight	170 kDa
Purity	≥95% as determined by SDS-PAGE
Purification	Immunogen affinity purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	liquid
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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NES, Nestin

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MCF7 cells were subjected to SDS PAGE followed by western blot with orb1100522 (NES antibody) at dilution of 1:1000.

Immunofluorescent analysis of SH-SY5Y cells using orb1100522 (NES antibody) at dilution of 1:50.

Immunohistochemistry of paraffin-embedded mouse brain using orb1100522 (NES antibody) at dilution of 1:50.

Quick Database Links

Gene Symbol

NES

UniProt

P48681

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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