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Nestin Mouse Monoclonal Antibody
SKU: orb1816769
Featured
Description
Images & Validation
−Item 1 of 3
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | IHC-P=1:100-500, WB=1:500-1000, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Human |
| Predicted Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a, κ |
| Clone No. | E2G4 |
| Immunogen | KLH conjugated synthetic peptide derived from human Nestin |
| Target | NES |
| Molecular Weight | 178 kDa |
| Purification | Affinity purified by Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS, Glycerol, rAlbumin. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_HUMAN; Nestin.
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Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Nestin) Monoclonal Antibody, Unconjugated (orb1816769) at 1:500 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Nestin) Monoclonal Antibody, Unconjugated (orb1816769) at 1:500 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Nestin) Monoclonal Antibody, Unconjugated (orb1816769) at 1:500 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Quick Database Links
Gene Symbol
NES
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
Nestin Mouse Monoclonal Antibody (orb1816769)
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