Osteopontin Rabbit Polyclonal Antibody

SKU: orb385622

Description

Osteopontin Rabbit Polyclonal Antibody is an unconjugated antibody that specifically recognizes Osteopontin. Generated against a KLH conjugated synthetic peptide derived from human Osteopontin. The peptide is available as orb13218. It is supplied in liquid form and exhibits reactivity with Human, Mouse and Rat samples. The suitable applications for this antibody are IHC-P and WB.

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

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Tested Applications	IHC-P, WB
Dilution Range	IHC-P: 1:50-500, WB: 1:200-1000
Reactivity	Human, Mouse, Rat

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Osteopontin. Please contact us for the exact immunogen sequence. The peptide is available as orb13218.
Target	Osteopontin
Molecular Weight	35 kDa
Purity	Polyclonal antibodies are purified by peptide affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	10 mM PBS, 0.02% sodium azide
Concentration	- 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

IHC-P staining of rat kidney tissue using Osteopontin antibody (2.5 ug/ml)

Immunohistochemical staining of human ovarian cancer tissue using anti-Osteopontin (2.5 ug/ml)

Western blot analysis of rat kidney (lane 1), mouse brain (lane 2), mouse testis (lane 3), human breast tumor (lane 4), human ovarian cancer (lane 5); u251 cells (lane 6) tissue using Osteopontin antibody (1 ug/mL)

Immunohistochemical staining of human ovarian cancer tissue using Osteopontin antibody (2.5 ug/ml)

Quick Database Links

Gene Symbol

Osteopontin

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Datasheet

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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