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PDCD10 Antibody

SKU: orb1274494

Description

PDCD10 Antibody

Research Area

Cell Biology, Neuroscience, Signal Transduction

Images & Validation

Tested ApplicationsIF, WB
ReactivityHuman, Mouse
Application Notes
WB: 1:200 - 1:2000IF: 1:50 - 1:200

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-212 of human PDCD10 (NP_009148.2).
TargetPDCD10
Molecular WeightObserved: 26kDa
PurificationAffinity purification
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Concentrationbatch dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Programmed cell death protein 10, Cerebral cavernous malformations 3 protein, TF-1 cell apoptosis-related protein 15, PDCD10, CCM3, TFAR15

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PDCD10 Antibody

Western blot analysis of extracts of various cell lines, using PDCD10 antibody (orb1274494) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

PDCD10 Antibody

Western blot analysis of extracts from normal (control) and PDCD10 knockout (KO) HeLa cells, using PDCD10 antibody (orb1274494) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IF
Immunofluorescence
View Protocol

PDCD10 Antibody (orb1274494)

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Available Sizes

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100 μl
$ 1,000.00
DispatchUsually dispatched within 5-10 working days
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