PDGF Receptor beta Recombinant Rabbit Monoclonal Antibody

SKU: orb2563055

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Description

Research Area

Cancer Research, Cell Biology, Growth Factors, Inflammation, Inflammatory Mediators, Platelet-derived growth factor, Receptor Tyrosne Kinases, SARS /COVID-19

Images & Validation

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Item 1 of 4

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:1000-5000, IHC-P=1:200-800, IF=1:200-800, IHC-F=1:200-800
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Mouse, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	3A3
Immunogen	A synthesized peptide derived from human PDGFR beta (1051-1106aa)
Target	PDGFRB
Molecular Weight	118 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Disclaimer	For research use only

Alternative Names

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Platelet-derived growth factor receptor beta; CD140B; beta platelet derived growth factor receptor; beta-type platelet-derived growth factor receptor; CD 140B; CD140 antigen-like family member B; CD140B; CD140b antigen; JTK12; OTTHUMP00000160528; PDGF R beta; PDGF-R-beta; PDGFR; PDGFR1; PDGFRB; PGFRB_HUMAN; Platelet derived growth factor receptor beta polypeptide; PDGF Receptor beta; beta-type platelet-derived growth factor receptor precursor;

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PDGF Receptor beta Recombinant Rabbit Monoclonal Antibody

25 ug total protein per lane of various lysates probed with PDGF Receptor beta monoclonal antibody, unconjugated (orb2563055) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded Human Glioma, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PDGF Receptor beta Monoclonal Antibody, Unconjugated (orb2563055) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PDGF Receptor beta Monoclonal Antibody, Unconjugated (orb2563055) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Uterus, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with PDGF Receptor beta Monoclonal Antibody, Unconjugated (orb2563055) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Quick Database Links

Gene Symbol

PDGFRB

UniProt

P09619

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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