Rabbit PDGFR beta Recombinant Monoclonal Antibody

SKU: orb1570943

Description

PDGFR beta Antibody

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Neuroscience

Images & Validation

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Tested Applications	FC, ICC, IP, WB
Dilution range	WB - 1:1,000; IP - 6 µl/1 mg lysate; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
Reactivity	Mouse
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	BLR242L
Immunogen	Residues 32-530 (ECD)
Target	PDGFR beta
Purification	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
Concentration	1000 µg/ml
Disclaimer	For research use only

Alternative Names

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AI528809; beta platelet-derived growth factor receptor; beta-type platelet-derived growth factor receptor; CD antigen CD140b; CD140 antigen-like family member B; CD140b; PDGF beta chain; Pdgfr; PDGFR-1; PDGF-R-beta; PDGFR-beta; platelet-derived growth factor receptor 1; platelet-derived growth factor receptor beta; platelet-derived growth factor receptor beta variant 1

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Rabbit PDGFR beta Recombinant Monoclonal Antibody

Detection of mouse PDGFR beta by western blot. Samples: Whole cell lysate (10 µg) from A20, NIH 3T3, C6, C2C12, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (orb1570943) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse PDGFR beta (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (orb1570943) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of mouse PDGFR beta by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PDGFR beta recombinant monoclonal antibody (orb1570943) used for IP at 6 µl/mg lysate.

Detection of mouse PDGFR beta by immunocytochemistry. Sample: FFPE section of mouse CT26 cells. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (orb1570943). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse PDGFR beta by immunocytochemistry. Sample: FFPE section of mouse C2C12 cells. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (orb1570943). Secondary: HRP-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

PDGFR beta

UniProt

P05622

RefSeq (Protein):NP_005745.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_005745.1

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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ICC

Immunocytochemistry

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Immunoprecipitation

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