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PEDF Rabbit Polyclonal Antibody

SKU: orb11261

Description

PEDF Rabbit Polyclonal Antibody

Images & Validation

Tested ApplicationsIF, IHC-Fr, IHC-P
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
ReactivityRat
Predicted ReactivityBovine, Canine, Human, Mouse, Porcine

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human PEDF (201-300/418aa)
TargetSERPINF1
Molecular Weight46 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

pigment epithelium-derived factor; Serpin-F1; Stromal cell-derived factor 3; SDF-3; Caspin; alpha 2 antiplasmin; EPC 1; EPC1; PIG 35; PIG35; Pigment epithelium derived factor; Proliferation inducing protein 35; SERPIN F1; Serpin peptidase inhibitor clade F member 1; SERPINF 1; SERPINF1; PEDF_HUMAN.

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PEDF Rabbit Polyclonal Antibody

Tissue/Cell: chorioid of rat eyes, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-PEGF Polyclonal Antibody, Unconjugated (orb11261) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

UniProt Details

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol

PEDF Rabbit Polyclonal Antibody (orb11261)

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Available Sizes

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50 μl
$ 240.00
100 μl
$ 390.00
200 μl
$ 510.00
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